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Title

Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems

Publication Year

2014

Author(s)
  • Bodeker, Inga T. M.
  • Clemmensen, Karina E.
  • de Boer, Wietse
  • Martin, Francis
  • Olson, Ake
  • Lindahl, Bjorn D.
Source
NEW PHYTOLOGIST Volume: 203 Issue: 1 Pages: 245-256 Published: 2014
ISSN
0028-646X eISSN: 1469-8137
Abstract

In northern forests, belowground sequestration of nitrogen (N) in complex organic pools restricts nutrient availability to plants. Oxidative extracellular enzymes produced by ectomycorrhizal fungi may aid plant N acquisition by providing access to N in macromolecular complexes. We test the hypotheses that ectomycorrhizal Cortinarius species produce Mn-dependent peroxidases, and that the activity of these enzymes declines at elevated concentrations of inorganic N. In a boreal pine forest and a sub-arctic birch forest, Cortinarius DNA was assessed by 454-sequencing of ITS amplicons and related to Mn-peroxidase activity in humus samples with- and without previous N amendment. Transcription of Cortinarius Mn-peroxidase genes was investigated in field samples. Phylogenetic analyses of Cortinarius peroxidase amplicons and genome sequences were performed. We found a significant co-localization of high peroxidase activity and DNA from Cortinarius species. Peroxidase activity was reduced by high ammonium concentrations. Amplification of mRNA sequences indicated transcription of Cortinarius Mn-peroxidase genes under field conditions. The Cortinarius glaucopus genome encodes 11 peroxidases - a number comparable to many white-rot wood decomposers. These results support the hypothesis that some ectomycorrhizal fungi - Cortinarius species in particular - may play an important role in decomposition of complex organic matter, linked to their mobilization of organically bound N.

Author Keyword(s)
  • carbon sequestration
  • Class II peroxidases
  • decomposition
  • ectomycorrhizal fungi
  • high throughput sequencing
  • nitrogen limitation
  • priming effect
  • transcription
KeyWord(s) Plus
  • SOIL ORGANIC-MATTER
  • DEGRADING HEME PEROXIDASES
  • MYCORRHIZAL FUNGI
  • BOREAL FOREST
  • C-14-LABELED LIGNIN
  • HIGH-THROUGHPUT
  • NITROGEN
  • DECOMPOSITION
  • CARBON
  • DEGRADATION
ESI Discipline(s)
  • Plant & Animal Science
Web of Science Category(ies)
  • Plant Sciences
Adress(es)

[Bodeker, Inga T. M.; Clemmensen, Karina E.; Olson, Ake; Lindahl, Bjorn D.] Swedish Univ Agr Sci, Dept Forest Mycol & Plant Pathol, S-75007 Uppsala, Sweden; [Bodeker, Inga T. M.] Swedish Univ Agr Sci, Southern Swedish Forest Res Ctr, S-23053 Alnarp, Sweden; [de Boer, Wietse] NIOO KNAW, Dept Microbial Ecol, NL-6700 AB Wageningen, Netherlands; [Martin, Francis] INRA, UMR 1136, F-54280 Champenoux, France

Reprint Adress

Lindahl, BD (reprint author), Swedish Univ Agr Sci, Dept Forest Mycol & Plant Pathol, Box 7026, S-75007 Uppsala, Sweden.

Country(ies)
  • France
  • Netherlands
  • Sweden
CNRS - Adress(es)
    Accession Number
    WOS:000336970200024
    uid:/JP70JDWP
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